No Significant Effects of Y-27632 on Cell Proliferation.

(A) Representative fluorescent images of cell proliferation evaluated with the EdU (5-ethynyl-2'-deoxyuridine) kit. EdU+ proliferative cells are shown in green (a4, a6, a7, & a9) and the corresponding nuclei were stained in blue (a5, a6, a8, & a9). No EdU positive cells were found in the Control-EdU group (a1 to a3). Scale bar = 200μm. (B) Display of the proliferation rates (%) measured with Flow Cytometry. Means of 3 separate measurements are plotted in the bar graphs below (n = 3, ns: no significance). No statistical significance was found between the Control+EdU and the Y-27632+EdU groups.