Effect of culture system on gene expression during traditional 2D culture and 3D culture of mouse mesothelioma cells

To investigate the differences between mesothelioma- organoid culture and 2D traditional culture, we established 7 mesothelioma lines and 3 normal mesothelial cells from model mouse. We then performed gene expression profiling analysis using data obtained from RNA-seq of 20 different cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for mesothelioma cells and its culture system between 2D and 3D.