Stroke target identification guided by astrocyte transcriptome analysis [pool]. Mus musculus
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA407000
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资源简介：
We aimed to investigate the influence of ischemic stroke in mice on their ribosomal mRNA in astrocytes. Therefore, we make use of the conditional Cre/LoxP System in the RiboTag mouse line in order to isolate the ribosomal mRNA of astrocytes. CreERT2 expression under the astrocyte-specific Cx43 promoter is induced by Tamoxifen injection and activates the expression of a haemagglutinin tag on ribosomal proteins. 3 weeks after Tamoxifen induction, mice were subjected to a 60 min middle cerebral artery occlusion (tMCAO), which is an established ischemic stroke model. Control mice were not further treated. Overall design: Pool from 3 animals in each group, 2 technical replicates each. From each sample RNA was extracted by IP two times and pooled back together.
创建时间：
2017-09-12
