Annotation of differentially expressed proteins identified in the endosperm and embryo of the three maize hybrids during the linear phase of grain filling.

aNumber of identified differentially expressed protein spots on each 2-D map. Preferentially accumulated proteins: more than 2-fold changes; t-test or ANOVA: PbAccession number of each protein identified by MS.cIdentified proteins obtained from the NCBI Zea mays protein sequence database using the TurboSEQUEST algorithm.dFunctional annotation of each protein identified by MS.eMolecular weight of protein on gel/predicted molecular weight of protein.fpI of protein on gel/predicted pI of protein.gNumber of peptides matching the corresponding protein.hCalculated by weighting ion score (based on the probability that ion fragmentation matches are non-random) events for all individual peptides matched to the protein. Ion scores for duplicated matches are excluded from the calculation.iConfidence level of the total ion score.jCalculated by Mascot search (−10×LogP) for identified proteins.kPercentage of coverage of the identified proteins.lProbability that the peptide mass matches are non-random events.