Methanosarcina mazei strain:Mm Mut 203b | isolate:Mm Mut 203b Genome sequencing and assembly. Methanosarcina mazei strain:Mm Mut 203b | isolate:Mm Mut 203b

Methanosarcina mazei Go1 wild type was transformed with plasmid pWM321 (Metcalf et al. 1997). A single positive colony was isolated, named Methanosarcina mazei mutant 203b (Mm Mut 203b), and used as an empty vector control during a long-term evolution experiment. As a reference for possible chromosomal mutations or rearrangements during the long-term experiment, strain Mm Mut 203b was sequenced using Illumina and Oxford Nanopore Sequencing and assembled using hybrid assembly.