RNA-sequencing of murine WT and MAZR-cKO FOXP3+ regulatory T cells (Treg cells)

The analysis of WT and MAZR-cKO mice indicates that the transcription factor MAZR plays an important role in Treg cell development and function. The aim of this NGS experiment was to determine the MAZR-dependent transcriptome in regulatory T cells to elucidate how MAZR controls Treg cell biology. The results of this genome-wide analysis indicate that MAZR-cKO Treg cells display only minor transcriptional differences, suggesting that MAZR has a minor role in establishing the transcriptional program in ex vivo analyzed Treg cells once the Treg cell fate has been acquired. Overall design: mRNA expression profiles of 6-8 week old splenic WT,DEREG and MAZR-cKO,DEREG (CD4-cre) mice were generated by deep sequencing.