Transcriptional profiling of mouse liver of PPARgamma null mice

We highlight sex dimorphism of NAFLD in a lipodystrophic mouse model obtained via total body deletion of PPARγ. The hepatic sex dimorphism is characterized by a more important hepatosteatosis in females compared to males, and is PPARγ- and sex-hormone-dependent. To characterize the molecular mechanisms underlying this phenotype microarray analysis of gene expression was performed in the liver of 7 and 20 week old mice, before and after the onset of the the hepatic sex-dimorphism. We examined 8 experimental groups: PpargΔ/Δ and control mice, male and females at 7 week and PpargΔ/Δ and control mice at 20 weeks. For each experimental group we analysed the transcriptional profile of 2/3 individual RNA from liver.