Transcriptomic analysis of primary human T cells and murine RAW 264.7 macrophages 3D cultured in different collagen densities

The density of the extracellular matrix has a dramatic impact on the gene expression profile of T cells and macrophages. This may reflect impaired immune response of T cells and macrophages in solid tumors. Overall design: Quantification of RNA expression in human PBMCs enriched for T cells through the selection of non-adhenernt cells and a murine macrophage cell line (RAW 264.7) cultured in different densities of extracellular matrix (Type I collagen). T cells were isolated from PBMCs