The effect of nitric oxide on intestinal tissue damage and erythrocyte membranes when modeling cardiopulmonary bypass and circulatory arrest

OBJECTIVES: to study the effect of exogenous nitric oxide on the degree of intestinal tissue damage and erythrocyte membranes when simulating cardiopulmonary bypass and circulatory arrest in an experiment. The study was carried out on sheep, n=24. The animals were divided into 4 equal groups of 6 in each: “CPB” and “CPB+NO”, “CPB+CA” and “CPB+CA+NO”. In the “CPB+NO”, “CPB+CA+NO” groups NO was delivered. In the “CPB” and “CPB+CA” groups, CPB and CA were performed without NO. During the experiment, we assessed the following: free hemoglobin, concentrations, dynamics of coefficient of microviscosity and polarity in the areas of lipid-lipid and protein-lipid interactions (CMPLI; CPPLI) of erythrocyte membranes. One hour after the completion of cardiopulmonary bypass, tissues of the small intestine were collected for subsequent morphometric analyses ( villus height, enterocyte height, the number of intraepithelial lymphocytes per 100 epithelial cells (In one biopsy of the small intestine, 5 elements (5 villi, 5 enterocytes, 5 the number of intraepithelial lymphocytes per 100 epithelial cells) were evaluated)). Perioperative delivery of exogenous NO during CPB and CA in the experiment reduces morphological signs of damage in intestinal tissue and has a positive effect on the structural organization of erythrocyte membranes. CPB-cardiopulmonary bypass; CA-circulatory arrest; NO-nitric oxide; CMLLI — coefficient of microviscosity in the areas of lipid-lipid interactions; CMPLI — coefficient of microviscosity in the areas of protein-lipid interactions; CPLLI — coefficient of polarity in the areas of lipid-lipid interactions; CPPLI — coefficient of polarity in the areas of protein-lipid interactions.