Mus musculus small RNA sequencing. Mus musculus

Next-generation sequencing (NGS) is the best way to comprehensively and quantitativelyanalysis exosomal RNA content and has been successfully applied to profile exosomal RNAfrom multiple cultured cells and human plasma. Mice are the most frequently usedanimals for basic and clinical studies. In the present study, we isolated and characterizedexosomes from mouse serum and profiled their small RNA contents by NGS. Moreover, basedon the obtained miRNA profile and existing knowledge regarding tissue and/or cell specificmiRNAs, we attempted to determine the potential sources of these circulating exosomes.Finally, the potential biological functions of mSEs were predicted by bioinformatics analysisand evaluated using in vitro experiments. These data will not only contribute to biomarkerstudies on serum exosomal miRNAs, but will also deepen our understanding of their biologicalsignificance.