Model parameters for reproducing fermentation.

The constraints that change through the iterative modifications in our attempt to recreate the experimental observations made during the fermentation. These correspond to the differences between the different panels in Fig 2. The various values given for lb refer to the lower bounds on the corresponding exchange reaction in the COBRA model, while the ub refer to the upper bounds. The exchange reactions are: ox, oxygen; lac, lactate; dha, dihydroxyacetone; ac, acetate; pyr, pyruvate. Where applicable, both stereoisomers are affected by the changes. The acetate excretion feedback (“ac feedback”) is described in the Results and discussion section.