Alignment of fiducial marks in a tomographic tilt series with an unknown rotation axis

Abstract
Alignment for tomography using a transmission electron microscopy frequently uses colloidal gold particles as fiducial reference marks. Typically, there is an implicit assumption that the tilt axis of the tomographic series is orthogonal to the beam direction. However, this may not be true, either intentionally, if a tilt-rotate stage is used, or unintentionally, because of mechanical errors in the rotation stage or the sample fixture. Here, we provide a computer code which takes as input a s...

Title of program: particleTilt
Catalogue Id: ADYW_v1_0

Nature of problem
The program is used to assist the alignment step in tomography. The samples should be prepared with spherical particles (typically gold beads) which are observed in several views. (Not every particle need be observed in every view.) The program reports coordinates of a 3D model of the particles as well as the direction of the tilt axis as a point on the unit sphere.

Versions of this program held in the CPC repository in Mendeley Data
ADYW_v1_0; particleTilt; 10.1016/j.cpc.2007.01.008

This program has been imported from the CPC Program Library held at Queen's University Belfast (1969-2019)

摘要
在采用透射电子显微镜进行断层扫描时，通常利用胶体金颗粒作为参考标记以实现对齐。通常存在一种隐含的假设，即断层扫描系列中的倾斜轴与束方向相互垂直。然而，这一假设可能并不成立，无论是由于采用了倾斜旋转台而有意为之，还是由于旋转台或样品夹具的机械误差而无意中导致。在此，我们提供了一款计算机代码，该代码以断层扫描系列为输入，...