Soil fungal necromass in deciduous-dominated boreal forest after 13 years of inorganic nitrogen addition

We applied inorganic N (30 kg ha-1 year-1) for 13 years (2006-2019) in a boreal forest dominated by Populus tremuloides Michx. and measured total fungal necromass concentrations (amino sugar; glucosamine) and total C concentrations in organic and mineral soil. We also measured total fungal biomass concentrations (ergosterol) in soil, condensed tannin and chitin concentrations of mycorrhizal roots, and the genetic capacity of the ectomycorrhizal fungal community to produce chitinases and class II peroxidases.

The experiment had four blocks, each containing two 20 × 20 m plots surrounded by a 5–10 m forest buffer. Each of the two plots within a block was randomly assigned to either control or N addition (30 kg N ha-1 year-1) treatment. Subsamples were taken from each of the plots and pooled.

This is an excel spreadsheet. The first tab describes the data and the two other tabs include the data.

These data are part of an existing dataset from the published paper Karst, J., Wasyliw, J., Birch, J. D., Franklin, J., Chang, S. X., & Erbilgin, N. (2021). Long-term nitrogen addition does not sustain host tree stem radial growth but doubles the abundance of high-biomass ectomycorrhizal fungi. Global Change Biology, 27(17), 4125–4138. https://doi.org/10.1111/gcb.15713

Average annual tree stem radial growth (mm) comes from tree ring analysis in Karst et al 2021 (above).

Total carbon in mineral soils comes from Kwak, J.-H., Naeth, M. A., & Chang, S. X. (2018). Microbial Activities and Gross Nitrogen Transformation Unaffected by Ten‐Year Nitrogen and Sulfur Addition. Soil Science Society of America Journal, 82(2), 362–370

The average number of glycoside hydrolase family 18 (GH18) and auxiliary activity family 2 (AA2) genes, respectively, among fungal genera using published values in the Mycocosm database:

Grigoriev, I. V., Nikitin, R., Haridas, S., Kuo, A., Ohm, R., Otillar, R., et al. (2014) MycoCosm portal: gearing up for 1000 fungal genomes. Nucleic Acids Research. 42, D699-D704. https://doi.org/10.1093/nar/gkt1183