Identification of immunodominant antigen among Brucella species (B. abortus and B. melitensis) using hyper immune serum from naturally infected hosts

In the present study, the whole cell protein extracts of B. abortus and B. melitensis were separated using SDS-PAGE and western blotting was carried out with the antiserum from naturally infected host animals (cow, buffalo, goat and sheep). The proteins bands that matched with western blot signals were excised, trypsin digested and subjected to MALDI identification.