Database of Ca2+ transients from single cell E. coli measurements
收藏资源简介:
Database configuration To efficiently compare conditions from over 7 Tb of image data, we constructed an HDF5 database with the extracted fluorescent traces for each experiment. The database was configured such that the fluorophore specifies the first group (e.g., '/gcamp'). The second group is the unique experimental identifier generated at the time of the experiment (e.g., '/20210322_5 Hz_gcamp_90pts'). The third group specifies the variable name (See Table below for description). The database is also available from https://bitbucket.org/meyerct6/soc_bacteria_repo/src/master/ in (Database/soc_bacteria_database.h5) and can be used to recreate most figures in the manuscript. Description of HDF5 database variables for each experiment /meanintMatG Matrix of mean intensity within each segmented cell (rows) at all time points (columns). /corrIntMatG Matrix of mean intensity corrected for photobleaching. /fitRsqr Vector of R-squared fit values for photobleach equation. /timeMat Matrix of acquisition times for each cell (rows) at every timepoint (columns). /heat_kill Boolean vector denoting whether the cell was heat killed



