Tensile strain and altered synovial tissue metabolism in human knee osteoarthritis

To investigate the physiologic responses of whole osteoarthritic synovium to cycle tensile strain. We obtained 3 matched synovial tissue samples from 6 patients undergoing total knee arthroplasty, subjected them to cyclic tensile strain, and then performed gene expression profiling analysis using RNA seq from each cyclic tensile strain protocol. Overall design: Comparative gene expression profiling analysis of RNA-seq data for whole osteoarthritis synovium subjected to 3 different cyclic tensile strain protocols (control (static), low-frequency (0.3Hz), and high-frequency (1.0Hz)).