MANF relieves cerebral infarction injury by enhancing the pyroptosis inhibition of BMSCs

Cerebral infarction is a common disease characterized by high mortality, narrow therapeutic window and limited therapeutic avenue.We constructed animal model of Middle Cerebral Artery Occlusion (MCAO), compared different expression genes (DEGs) between Sham and MCAO group by single-cell RNA sequencing (scRNA-seq) to explore the potential cell death-related pathways involved in cerebral infarction.We found that the DEGs up-regulated in four major cellclusters after MCAO were enriched for not only apoptosis, ferroptosis and necroptosis, but also pyroptosis-related pathways and functions. Surprisingly, our protein-protein interaction (PPI) networks analysis showed indirect correlation between MANF and pyroptosis-related proteins. Overall design: Examination of 2 groups of Sham and MCAO, we mixed 5 samples per group into one cell suspension pools.