Transcriptomes of UFMylation gene knockdown in human breast cancer cell. Transcriptomes of UFMylation gene knockdown in human breast cancer cell

To investagate the gene expression profiling of dysUFMylation in breast cancer, we constructed UFL1 and UFM1 konckouted-MDA-MB-231 using CRISPR-Cas9 and couducted RNA-seq. mRNAs were isolated and purified using RNeasy Plus Mini Kit (Qiagen). Samples were prepared from three biological replicates. Overall design: Total RNA transcriptomes of UFL1 or UFM1 KO MDA-MB-231 cells were profiled by RNA-seq.