Genomic copy number variation induced by TAQing system in Saccharomyces cerevisiae.. Saccharomyces cerevisiae

We developed an artificial genome evolution system, which we termed ‘TAQing’, by introducing multiple genomic DNA double-strand breaks using a heat-activatable endonuclease in mitotic yeast. The heat-activated endonuclease, TaqI, induced random DSBs, which resulted in diverse types of chromosomal rearrangements including translocations. Array comparative genomic hybridization (aCGH) analysis was performed with cell-fused Saccharomyces cerevisiae strains induced genome evolution by TAQing system. Some of copy number variations (CNVs) induced by massive genome rearrangements were detected in the TAQed yeast strains. Overall design: We generated 13 mutrant strains with CNVs using TAQing system in yeast. We analyze contorol strain (WT14) and 13 mutants genome using aCGH, and detected CNVs in these mutants genome as compared to WT14 genome.