Data from: Tolerance to gamma radiation in the tardigrade Hypsibius dujardini from embryo to adult correlate inversely with cellular proliferation

Tardigrades are highly tolerant to desiccation and ionizing radiation but
the mechanisms of this tolerance are not well understood. In this paper,
we report studies on dose responses of adults and eggs of the tardigrade
Hypsibius dujardini exposed to gamma radiation. In adults the LD50/48h for
survival was estimated at ~ 4200 Gy, and doses higher than 100 Gy reduced
both fertility and hatchability of laid eggs drastically. We also
evaluated the effect of radiation (doses 50 Gy, 200 Gy, 500 Gy) on eggs in
the early and late embryonic stage of development, and observed a reduced
hatchability in the early stage, while no effect was found in the late
stage of development. Survival of juveniles from irradiated eggs was
highly affected by a 500 Gy dose, both in the early and the late stage.
Juveniles hatched from eggs irradiated at 50 Gy and 200 Gy developed into
adults and produced offspring, but their fertility was reduced compared to
the controls. Finally we measured the effect of low temperature during
irradiation at 4000 Gy and 4500 Gy on survival in adult tardigrades, and
observed a slight delay in the expressed mortality when tardigrades were
irradiated on ice. Since H. dujardini is a freshwater tardigrade with
lower tolerance to desiccation compared to limno-terrestrial tardigrades,
the high radiation tolerance in adults, similar to limno-terrestrial
tardigrades, is unexpected and seems to challenge the idea that
desiccation and radiation tolerance rely on the same molecular mechanisms.
We suggest that the higher radiation tolerance in adults and late stage
embryos of H. dujardini (and in other studied tardigrades) compared to
early stage embryos may partly be due to limited mitotic activity, since
tardigrades have a low degree of somatic cell division (eutely), and
dividing cells are known to be more sensitive to radiation.