QTL mapping: insights into genomic regions governing component traits of yield under combined heat and drought stress in wheat

The mapping population comprises of 180 RILs developed from a cross between GW322 and KAUZ.
Phenotypic dataPhenotypic evaluation was conducted across two consecutive crop seasons (2021-22 and 2022-23) under late sown irrigation (LSIR) and late sown restricted irrigation (LSRI) conditions at ICAR-IARI, New Delhi. Various physiological and agronomic traits of importance were measured. The component traits of yield including days to heading (DH), normalized difference vegetation index (NDVI), SPAD chlorophyll content (SPAD), plant height (PH), spike length (SL), thousand-grain weight (TGW), grain weight per spike (GWPS), biomass (BM) and grain yield per plot (PY) were measured under heat and combined stress conditions.
Genotypic dataDNA was isolated from 21-day-old seedlings using the CTAB method (Murray and Thompson, 1980). DNA quality check was done using 0.8% agarose gel electrophoresis. The Axiom Breeders' array containing 35K Single Nucleotide Polymorphism (SNP) was employed for the g..., The experiment was carried out at ICAR-IARI, New Delhi (28.6550° N, 77.1888° E, MSL 228.61 m) over two consecutive crop seasons (2021-22 and 2022-23). The study involved the evaluation of RILs developed from GW322/KAUZ along with their parents under two distinct conditions: heat stress (late sown irrigation; LSIR) and combined drought and heat stress (late sown restricted irrigation; LSRI) in augmented design (Federer, 1956; Federer, 1961; Searle, 1965). The experimental field was divided into four blocks, with four checks utilized in the study. Each check was replicated three times.
Phenotyping:The instrument GreenSeeker™ was used to record NDVI. NDVI was measured at four stages: at anthesis, 10 days after anthesis, 20 days after anthesis and 30 days after anthesis. To record TGW, manual counting of grains was followed and the weight of the grains was recorded in grams with an electronic balance. Days to heading is the number of days from sowing to 50% heading is recorded for each geno..., , # QTL mapping: insights into genomic regions governing component traits of yield under combined heat and drought stress in wheat

Author/Principal Investigator Information
Name: Dr Harikrishna
ORCID:
Institution: ICAR-Indian Agricultural Research Institute, New Delhi, India
Address: ICAR-Indian Agricultural Research Institute, New Delhi, India-110012
Email:
Date of data collection: 2021-12-01 TO 2023-07-31
Geographic location of data collection: ICAR-Indian Agricultural Research Institute, New Delhi (28°38′30.5″N, 77°09′58.2″E, 228 m AMSL)

Information about funding sources that supported the collection of the data: Part of the research was supported by a grant from Bill & Melinda Gates Foundation (Grant number # OPP1215722) sub-grant to India for Zn mainstreaming project and National Innovations on Climate Resilient Agriculture (NICRA) a network project of the Indian Council of Agricultural Research (ICAR).

## Description of the data and file structure

### File List:

Phenoty...