Genome-wide RNA-seq from c-MYC-depleted and rescued MDA-MB-231BrM cells

Purpose: To identify downstream signaling pathways that mediate functions of c-MYC Methods: RNAs isolated from MDA231-BrM cells expressing shCntr or shc-MYC, pCDH-puro control vector or c-MYC expression vector were analyzed by using an Illumina Nextseq500 Conclusions: Our study represents the first transcriptome profile of c-MYC-depleted and rescured MDA231-BrM Overall design: mRNAs prepared from c-MYC knockdown or c-MYC rescued MDA-MB-231 BrM cells were analyzed in duplicaters by Illumina Nextseq500. RNA-Seq reads were aligned to the human reference genome (NCBI 38) using the spliced read aligner STAR software (ver2.5)