Gut microbial variation in lactose intolerance mice with intervention of synthetic bacteria

In this study, we propose engineered probiotics to enhance the adjustability and controllability of LI treatment. With the strength of synthetic biology, next generation of probiotics in human body have been possible, with functions as eradication of infectious disease and cancer diagnostics. A tri-stable gene circuit was used in our engineered probiotics which could response to and improve LI symptoms in gastrointestinal tract. The enzyme activity and pH rescue function were characterized, followed by comprehensive analysis of the gut microbiome in BALB/c mice after lactose challenge and probiotics treatment. Noticeably, our engineered probiotics showed efficient functionality in vitro and in vivo.