Gene profilling of splenic CD4+ T cells from naive H2-O Wildtype and H2-O Knockout mice

Loss of the MHC Class II accessory molecule H2-O has been shown to alter the peptide repertoire presented by MHC Class II molecules. Using scRNA-Seq we interrogated what outcomes altered antigen presentation was having on the naive CD4 T cell milieu. CD3+ CD4+ (B220/NK1.1 negative) T cells were FACS sorted from the spleens of 3 H2-O Wildtpe and 3 H2-O knock-out mice and analyzed by scRNA-Seq analysis.