Supporting data for "The role of Centrosomal Protein of 295 kDa (CEP295) in cellular function and hepatocarcinogenesis"

The data set imcludes raw data of qPCR results, flowcytometry analysis, mass spectrometry results and microscopy images supporting the data interprited in "The role of Centrosomal Protein of 295 kDa (CEP295) in cellular function and hepatocarcinogenesis".

Eight trials of qPCR CT value data were included for the analysis of CEP295 transcript level in various of HCC cell lines, upon siRNA transfections and local clinical cohort samples. A general centrosomal protein transcript level screening results were also included up siRNA transfection and collected at 8 hour and 24 hour respectively.

Protein profile metadata renderred by HKU CPOS on cell fractionated samples were stored under the folder of "massSpec". The raw profile plots and downstream proteomic library matching and exact peptide sequence match were included, for individual file description please refer the "README.txt" file.

The exported flow cytometry raw data covered the time course DNA profile experiment on siRNA transfected HCC cells from 0 hour to 6 hour in one-hour interval collection. 

The images on microtubule aster formation assay on control and siRNA transfected HCC cells were located under "confocal" folder. Cells were fixed and stained with described the targets and harvested at indicated time points. The images were separated in individual wavelength channel and merged channel for better understanding of the target proteins subcellular localization.

本数据集囊括了支持《295 kDa 中枢蛋白（CEP295）在细胞功能及肝细胞癌发生发展中的作用》一文中解读的原始数据，包括 qPCR 结果、流式细胞术分析、质谱分析数据以及显微镜成像。数据集分析了八次 qPCR CT 值试验，涉及不同肝细胞癌（HCC）细胞系在 siRNA 转染及临床队列样本中的 CEP295 转录水平，并包含了 siRNA 转染后的普遍性中枢蛋白转录水平筛查结果，分别在8小时和24小时收集。香港大学CPOS中心在细胞组分样本上生成的蛋白质概况元数据存储于“massSpec”文件夹中，包括原始轮廓图、下游蛋白质组库匹配以及精确肽序列匹配，具体文件描述请参阅“README.txt”文件。导出的流式细胞术原始数据涵盖了siRNA转染的HCC细胞从0小时至6小时的DNA轮廓实验，每隔一小时收集一次。位于“confocal”文件夹中的图像展示了控制组和siRNA转染的HCC细胞微管星形结构形成实验，细胞在指定时间点固定并标记目标蛋白，图像根据不同波长的单通道和合并通道进行分离，以便更好地理解目标蛋白的亚细胞定位。