SPP1+ Macrophages Contribute to Hair Growth in Contact Hypersensitivity [bulk RNA-Seq]

Transcriptomic analysis of sorted HFSCs revealed dynamic transcriptional reprogramming during inflammation, including activation of stress response and immune-modulatory genes. Pathway enrichment highlighted metabolic adaptation and signaling networks linked to stem cell activation. These findings demonstrate that HFSCs undergo intrinsic molecular changes that parallel their regenerative response. Overall design: We sorted hair follicle stem cells (HFSCs) from day2 DPCP-treated skin and day0 control skin using flow cytometry based on established surface markers. Cell populations were sorted directly into TRIzol LS Reagent (Invitrogen). RNA was isolated using Direct-zol RNA Miniprep Kits. Purified RNA were subjected to transcriptomic profiling to uncover molecular signatures and regulatory pathways underlying stem cell activation during skin inflammation.