Metabolic maturation of neonatal hearts driven by ?-linolenic acid in maternal milk [RXR ChIP-seq]

The goal of this study was to compare the cistrome profile of RXR (ChIP-seq) of perinatal hearts of cardiomyocyte-specific RXRab-deficient and WT mice, in order to identify genomic regions which are directly controlled by TF RXRa and RXRb. Overall design: P0 hearts from wild type (WT) and cardiomyocyte-specific RXRab-/- (edKO) newborns were collected and analyzed. Individual P0 hearts were considered as individual biological replicates. Prior to cardiac tissue homogenization, each heart was sequentially fixed with DSG and FA. Chromatin was extracted and then sheared using a Covaris sonicator. Immunoprecipitation was performed with the corresponding antibody and Dynabeads A. Input was obtained by keeping a 10% total chromatin.