Identification of LINC00152 target genes in comparison in A172 and U87 glioblastoma cells

The long noncoding RNA LINC00152 shows ubiquitous expression and is often upregulated in tumor entities compared to healthy tissues. LINC00152 promotes malignant progression in the glioblastoma cell line U87. Here, LINC00152 knockdown leads to a reduction of migration and invasion of tumor cells. However, LINC00152 seems to have an opposite effect in another glioblastoma cell line A172. For this reason, the transcriptional patterns after LINC00152 knockdown in both cell lines (U87 and A172) were compared to identify the differences. Gene expression profiles (all protein-coding- and several important non-coding transcripts) after LINC00152 knockdown in the human glioblastoma cell lines A172 and U87 were measured 40 hours after transfection with either a negative control siRNA and an siRNA to LINC00152 (exon1). Three independent biological experiments for A172 and four independent biological experiments for U87 were performed for each siRNA approach, yielding 14 approaches in total.