Mechanistic study of Wee1 kinase inhibition with AZD1775 exposes drug targetable vulnerabilities in acute B-lymphoblastic leukemia [scMultiome]

This study characterized the effect of WEE1 kinase inhibition using AZD1775 treatment on single-cell accessible chromatin and gene expression profile using the 10x Genomics multiome protocol (ATAC + Gene Expression Assay) in the acute lymphoblastic leukemia cell line RS4;11 that represents the KMT2A-rearranged subtype. Overall design: scMultiome-seq samples were generated from cells treated for 10 h with AZD1775(Wee1i) or vehicle (DMSO) using the 10x Genomics Chromium platform and protocols generating scRNA-seq and scATAC-seq from the same cells.