De novo specification of epidermal cells in abscission zone furthers post-abscission cell growth

Single-cell RNA-seq (scRNA-seq) analysis using REC protoplasts obtained from the transgenic plants harboring prEXL1:nls-GFP. The AZ cells at flower development stages 15 to 17, which comprise before and after the completion of abscission, were protoplastated and subjected to fluorescence-activated cell sorting to collect GFP-positive cells. Approximately 100,000 protoplasts were immediately subjected to the droplet-based scRNA-seq analysis to acquire 7,000 single-cell transcriptomes