HPLC-ESI-MS characterization of phytochemicals in Sarghina extract

HPLC analysis of sarghina extract was performed on the Dionex Ultimate 3000 HPLC System (Thermo Fisher Scientific, USA), equipped with the Ultimate 3000 RS Variable Wavelength UV DAD. DAD was performed in-line in UV–VIS spectra in the range of 200–800 nm. A column (Acclaim Polar Advantage 5µm - C18 - 250 mm x 4.6 mm) was used and the oven temperature was maintained at 30°C. Data acquisition was performed by Xcalibur software (version 3.1, Thermo Fisher Scientific).