DataSheet1_Pb2+ Responsive Cu-In-Zn-S Quantum Dots With Low Cytotoxicity.pdf

Water-soluble Cu-In-Zn-S quantum dots (CIZS QDs) with orange fluorescence have been synthesized with a glutathione (GSH) as stabilizer via facile a one-step hydrothermal method. The optimal reaction conditions of CIZS QDs including temperature, time, pH, and the molar ratios of precursors were studied. TEM results indicate that the aqueous-dispersible CIZS QDs are quasi-spherical, and the average diameters are 3.76 nm with excellent fluorescent stability. Furthermore, the cytotoxicity of CIZS QDs was investigated by the microcalorimetry combining with TEM and the IC50 was 10.2 μM. CIZS QDs showed a promising perspective in applications such as a fluorescent probe for bioimaging and biolabeling due to the low cytotoxicity and good biocompatibility. Moreover, the CIZS QDs can distinguish Pb2+ ion from other ions, offering great potentials in lead ion determination in drinking water. According to the results of UV, XRD, FL, PL, and ITC methods, the mechanism of CIZS QDs-Pb2+ assay is due to hydrogen bonding or van der Waals forces in the formation of Pb2+ and CIZS QDs.

采用甘肽（GSH）作为稳定剂，通过简便的一步水热法合成了具有橙色荧光的水溶性Cu-In-Zn-S量子点（CIZS QDs）。对CIZS QDs的最佳反应条件，包括温度、时间、pH值以及前驱体的摩尔比进行了研究。透射电子显微镜（TEM）结果显示，水溶性CIZS QDs呈准球形，平均直径为3.76 nm，并具有优异的荧光稳定性。此外，通过结合微热量计、TEM和IC50值10.2 μM的细胞毒性研究，探讨了CIZS QDs的细胞毒性。CIZS QDs因其低细胞毒性和良好的生物相容性，在生物成像和生物标记荧光探针等应用领域展现出巨大的应用前景。进一步地，CIZS QDs能够区分Pb2+离子与其他离子，为饮用水中铅离子测定提供了巨大的潜力。根据紫外光谱（UV）、X射线衍射（XRD）、荧光光谱（FL）、光致发光（PL）和量热法（ITC）的结果，CIZS QDs-Pb2+检测的机制归因于Pb2+与CIZS QDs形成过程中的氢键或范德华力。