Functional characterization of NSE4A subunit of SMC5/6 complex in Arabidopsis

The control of genome integrity throughout cellular generations is critical for plant development and the correct transmission of genetic information to the progeny. A key factor involved in this process is STRUCTURAL MAINTAINANCE OF CHROMOSOME 5/6 (SMC5/6) complex, related to cohesin and condensin complexes. Here, we analyzed mRNA abundance changes in the partial loss of function mutant of NON-SMC ELEMENT 4A (nse4a-2 mutant allele), a subunit of SMC5/6 complex, in Arabidopsis. Overall design: Arabidopsis Col-0 wild-type and nse4a-2 plants were grown under control and zebularine stress conditions, their shoot apices were dissected and analyzed for mRNA abundances using RNA-sequencing.