Transcriptomic profiling of AR knockdown in human triple-negative breast cancer Hs578T cells

This study aimed to investigate transcriptomic alterations induced by androgen receptor (AR) silencing in the radioresistant Hs578T triple-negative breast cancer (TNBC) cells. Using siRNA-mediated knockdown of AR, RNA sequencing was performed to identify genes and pathways associated with AR signaling in radioresistant TNBC cells. Comparative transcriptomic profiling between control and AR-silenced cells revealed significant changes in pathways related to DNA damage response, apoptosis, and mTOR signaling. These data provide insights into the molecular mechanisms underlying AR-mediated regulation in TNBC and potential targets for therapeutic intervention.