Gene expression profiling of mouse pancreatic tumors derived from acinar or ductal cells

Acinar and ductal cells are the major epithelial cell types in the exocrine pancreas, but which of these serves as the cell-of-origin in human PDAC has been debated. Our mouse models have demonstrated that oncogenic Kras expression and Trp53 loss in pancreatic acinar or ductal cells can lead to pancreatic cancer. Here, we performed gene expression profiling of bulk acinar cell-derived and ductal cell-derived mouse pancreatic tumors to identify the transcriptomic profiles. Overall design: RNA-Seq was performed on 7 tumors originating from mouse models of acinar cell-derived (KT;Ptf1aCreER;Trp53fl/fl) or ductal cell-derived (KT;Sox9CreER;Trp53fl/fl) pancreatic cancer. KT = KrasLSL-G12D/+;R26LSL-tdTom/LSL-tdTom