In vivo imaging of subcellular structures and gene expression in mouse abdominal organs with the microstage device.

(A–D) Highly methylated chromocenters labeled by mRFP–MBD-nls in cells of liver (A), kidney (B), pancreas (C) and skeletal muscle (D) of mRFP–MBD-nls transgenic mice. Green pseudocolor shows the vasculatures labeled with an intravenous injection of AngioSense 750 IVM. Insets: magnified pictures of the boxed area in corresponding images. (E) Quantitative analysis of chromocenter numbers in cell nuclei of four types of tissues. Counts of 24, 23, 23 and 32 cell nuclei were analyzed for the liver, kidney, pancreas and skeletal muscle, respectively. (F–I) Autophagy in pancreatic acinar cells (F, G) and skeletal muscle (H, J) of GFP–LC3 mice before (F, H) and after (G, I) 24 h starvation. (J) Quantitative analysis of autophagy in response to 24 h starvation. The numbers of GFP–LC3 dots were counted and divided by the corresponding area (n = 5 image fields). (K–R) Fluorescence intensity in liver (K, O), kidney (L, P), pancreas (M, Q) and skeletal muscle (N, R) of ERAI-transgenic mice injected with saline (K–N) or tunicamycin (O–R). (S) Quantitative analysis of fluorescence intensity in tissues under different physiological conditions (n = 3 image fields). Results in E, J and S represent the mean ± SD. **P