A Zebrafish JAK1-A634D Gain-of-Function Model Provides New Insights into the Pathogenesis of Familial Hypereosinophilia

JAK1 plays an important role during zebrafish development as hematopoietic regulator with a specific impact in the myeloid lineage, from where the eosinophils arise. Several genes in the JAK-STAT pathway are elevated in the transgenic mutants (STAT1B, SOCS3A, SOCS3B, PIAS2) as well as patways involved in hematopoiesis, immunity, response to interferon and response to biotic stimuli. Total RNA from casper, JAK1-WT and JAK1-A634D zebrafish embryos at 28 and 36 hours post-fertilization was extracted to characterize the transcriptomic effects of the JAK1-A634D gain-of-function mutation