Lactobacillus brevis strains UCCLBBS124 and UCCLBBS449, control (MRS broth) vs. stress condition (5 % EtOH, pH4 or 30 ppm iso-a-acids)

Transcriptional profiling of Lactobacillus brevis UCCLBBS124 and UCCLBBS449 comparing control strain grown in MRS broth with strains growing in different stress conditons (5 % EtOH, pH4 or 30 ppm iso-a-acids).This study aimed to evaluate how certain Lb. brevis isolates are adapted so as to allow them to survive and grow in beer. DNA-microarrays containing oligonucleotide primers combining shared and unique genes of the 2648 and 2719 annotated genes on the genome of Lb. brevis UCCLBBS124 and UCCLBBS449, respectively, were designed by and obtained from Agilent Technologies (Palo Alto, Ca., USA). Three diffrerent experiments: MRS broth vs. MRS broth 5% EtOh, MRS broth vs. MRS broth pH4, MRS broth vs. MRS broth 30 ppm iso-a-acids for each of the two Lb. brevis strains UCCLBBS124 and UCCLBBS449