Affinities (KD) and kinetic parameters (ka and kd) for ABDTNF1, ABDTNF2 and non-randomized ABD.
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https://figshare.com/articles/dataset/_Affinities_K_D_and_kinetic_parameters_k_a_and_k_d_for_ABD_TNF1_ABD_TNF2_and_non_randomized_ABD_/399584
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(*)Saturation was not reached for the highest concentration (20 µM).Binding to HSA and TNF-α was determined by surface plasmon resonance spectroscopy (SPR) or on-cell by flow cytometry. Kinetic constants from SPR-measurements were measured on duplicate samples and KD calculated from the mean values. On-cell measurements were performed once for HSA and in duplicates for TNF-α. All parameters for TNF-α are calculated from the concentration of monomeric protein.
创建时间：
2015-12-02
