Salivary proteome signatures in the early and middle stages of human pregnancy with term birth outcome

This prospective study is to find changes in salivary protein expressions from first to early third trimester of pregnancy in normal term birth using SWATH-MS strategy. Saliva at three periods of gestation, 6-13 (V1), 18-21(V2), and 26-29 (V3) weeks from 20 singleton pregnant women were used in a discovery phase. Selected proteins from the discovery study were verified by targeted schedule-MRM (multiple reaction monitoring) experiments in a separate subset of subjects (N=14). Using in-house generated saliva-specific protein library, 65 proteins (q-value<0.1) changed as a function of gestational age.