RNA-seq analysis of gene expression after exposure to hot and cold stress in WT cells of Thermosynechococcus elongatus, BP-1

To investigate the temperature response in Thermosynechococcus elongatus (BP-1) and look for coordinated control in the cell, transcriptomes of BP-1 were measured using RNAseq following exposure to low and high temperature stress. The amount of temperature increase (53 to 61 °C) and decrease (53 to 45 °C) was based on the allowable range of continuous growth. The cells were growth in three separate culture tubes under LED light in a temperature-controlled submersible chamber under batch growth conditions. The temperature shift was conducted once the cells reached their target concentration of 1.5E7 cells/ml. A control experiment was conducted, in which the temperature remained at 53 °C, ensuring that the cellular physiology and light attenuation were comparable and that the only difference between the hot and cold treatments relative to the control treatment was temperature. Overall design: Heat stress 61°C and cold stress 45°C samples taken at time 0, 0.5hr, 3hr, 10hr, and 24hr. A control experiment, in which the temperature remained at 53 °C samples taken at time 3hr, 10hr, and 24hr. Each sample timepoint consisted of three biological replicates.