Large-scale stringent purification of HTT-HAP40 using heparin affinity chromatography 2019/05/12

<strong>Project: </strong>Biophysical investigation of purified HTT protein samples <strong>Experiment: </strong>Large-scale stringent purification of HTT-HAP40 using heparin affinity chromatography <strong>Date completed:­ </strong>2019/05/12 <strong>Rationale: </strong>Previous attempts to generate a much purer and homogenous HTT-HAP40 sample showed that the complex can bind heparin resin. In small-scale experiment, I also incorporated helpful suggestions from scientists at the CHDI Palm Springs meeting full-length HTT research breakout group i.e. ATP wash to remove HSP proteins. The experiment included 3 affinity chromatography steps with FLAG, heparin and NiNTA resin and finally a gel filtration step and yielded a highly pure HTT-HAP40 sample: https://zenodo.org/record/2628064.