Deep sequencing datasets from: RNA-catalyzed evolution of catalytic RNA

This dataset includes raw and processed sequencing data from evolving RNA populations described in Nikos Papastavrou, David P Horning, Gerald F Joyce. \"RNA-Catalyzed Evolution of Catalytic RNA\" (submitted). Briefly, directed evolution of a hammerhead ribozyme sequence was carried out over eight rounds of three steps each: 1) templated synthesis of the reverse-complement hammerhead RNA by a polymerase ribozyme; 2) templated synthesis of a new copy of the hammerhead RNA from the reverse-complement by a polymerase ribozyme; 3) selective recovery of hammerhead RNA that cleaved an attached RNA substrate. Cleaved RNA was reverse transcribed, PCR amplified, and archived for sequencing, while a portion was in vitro transcribed with T7 RNA polymerase to initiate the next round of evolution. Two distinct branches of evolution were carried out for 8 rounds, using the '52-2' or '71-89' polymerase ribozymes to replicate RNA, respectively. Sequenced RNA populations were analyzed to determine polymera..., Directed evolution of a hammerhead ribozyme sequence was carried out in three steps: 1) templated synthesis of the reverse-complement hammerhead RNA by a polymerase ribozyme; 2) templated synthesis of a new copy of the hammerhead RNA from the reverse-complement by a polymerase ribozyme; 3) selective recovery of hammerhead RNA that cleaved an attached RNA substrate. Cleaved RNA was reverse transcribed, PCR amplified, and archived for sequencing, while a portion was in vitro transcribed with T7 RNA polymerase to initiate the next round of evolution. Two distinct branches of evolution were carried out for 8 rounds, using the '52-2' or '71-89' polymerase ribozymes to replicate RNA, respectively. A third 'reselection' branch took six distinct hammerhead ribozyme sequences, and subjected them to a single round of directed evolution either individually or as an equimolar mixture, using the '71-89' polymerase for RNA replication.
RNA products from each of three steps of each round of evolution ..., , # Deep sequencing datasets from: RNA-Catalyzed Evolution of Catalytic RNA.

This dataset includes raw and processed sequencing data from evolving RNA populations described in Nikos Papastavrou, David P Horning, Gerald F Joyce. \"RNA-Catalyzed Evolution of Catalytic RNA\" (submitted). DNA libraries from each round of selection were prepared as described in the \"Materials and Methods\" and \"Supplementary Information: Supporting Text\" sections of that manuscript. Briefly, directed evolution of a hammerhead ribozyme sequence was carried out in three steps: 1) templated synthesis of the reverse-complement hammerhead RNA by a polymerase ribozyme; 2) templated synthesis of a new copy of the hammerhead RNA from the reverse-complement by a polymerase ribozyme; 3) selective recovery of hammerhead RNA that cleaved an attached RNA substrate. RNA products from each of these three steps ('HHR-', 'HHR+ pre-cleaved', and 'HHR+ cleaved', respectively) were reverse transcribed, amplified by PCR, and seque...