Transcriptomic profiling of HEK293 cells during adaptation from adherent to suspension culture

Human embryonic kidney 293 (HEK293) cells are a critical platform for the production of viral vector-based vaccines. While adapting adherent cells to suspension culture is a common industrial process, the underlying molecular mechanisms remain unclear.In this study, we performed high-throughput RNA sequencing (RNA-seq) to investigate the transcriptomic landscape of HEK293 cells during the transition from serum-dependent adherent growth to serum-free suspension culture. Samples were collected at three key stages: (1) parental adherent cells in 10% FBS, (2) intermediate cells during stepwise serum reduction, and (3) fully adapted suspension cells.The goal of this project is to identify differentially expressed genes (DEGs) and enriched pathways associated with cytoskeletal remodeling, metabolic reprogramming, and stress resistance. This data supports a multi-omics study aimed at rationally designing robust cell factories for efficient adenoviral vector production.