Human Memory Cell Sequencing. Homo sapiens

B cells have been segregated into distinct phenotypic and functional populations based on expression of cell surface markers. Amongst these heterogeneous populations, CD11c+, CD27- memory B cells, also known as atypical memory B cells (atBCs), accumulate in large numbers in conditions of autoimmunity such as systemic lupus erythematosus (SLE) or chronic infections like malaria or HIV. To provide a transcriptional basis for defining distinct B cell subsets and better understand the role of atBCs we performed single cell RNA-seq using both the Smart-seq 2 protocol and 10x Chromium platform to analyse circulating IgD- B cells from healthy human control subjects or subjects exposed to malaria infection.