Comparison of CD4+ and CD8+ T cells from choroid plexus of mice raised in standard or in enriched environment.. Mus musculus

C57BL6/J female mice were raised either in standard environment (SE) for 8 weeks after birth or were put in enriched environment (EE) at postnatal 4 weeks up to 8 weeks old. Mice were sacrificed at 8 weeks and choroid plexus in the brain were harvested. CD4+ and CD8+ T cells were sorted by FACS and 5-20 CD4+ or CD8+ T were profiled using a low-input protocol adapted from single-cell RNAseq. Overall design: 31 samples were profiled: for CD8+ T Cells, n=7 in EE and n=7 in SE. For CD4+ T Cells, N=9 in EE and N=8 in SE.