Analysis of estrogen-induced mRNA in endometrial cancer cells with forced expression of ER

We used the endometrial adenocarcinoma cell line in which the ERα cDNA gene was transfected and forcedly expressed ER. Then the factors induced by estrogen (Estradiol: E2) were measured by microarray. Ishikawa cells were transfected ERα cDNA gene. After 24hr hours , E2 was added at a concentration of 1X10-7 M or the same amount of ethanol was added to the control group. RNA was recovered from cells 24h, 48h and 72h after E2 was added, and comprehensive analysis was performed by microarray.