Effects of sequential combination of 5-FU with cDDP and DENSpm on SQ values in 2008 and C13* cells.

Synergism of growth inhibition was determined by treatment of cells with 5-FU, DENSpm and cDDP alone and in sequential combination where the first drug was added at Day 1 and the second drug was added at Day 2. Counting the cell biomass was done at Day 4. Synergism Quotients (SQ) have been calculated as reported in material and methods. The concentration of 5-FU was chosen to obtain values for percentage growth inhibition no greater than 30% when added alone. Statistical significance was estimated by two-tailed paired Student's t-test comparing the samples where 5-FU was added before the other drug with the samples where 5-FU was added after the other drug. (a,b,c,d P