Genome-wide maps of LDL1pro::LDL1:GFP in 14 days old arabidopsis

We report the application of single-molecule-based sequencing technology for high-throughput profiling of H3K4 demethylase LDL1 in 14 days old arabidopsis. By obtaining sequence from chromatin immunoprecipitated DNA, we generated genome-wide LDL1-binding maps of 14 days old arabidopsis. To reveal bound genes by LDL1, chimeric protein LDL1-GFP was expressed under LDL1 promoter in ldl1 (LDL1pro:LDL1:GFP/ ldl1). ChIP was performed using anti-GFP antibody (ab290; ABCAM), and ChIP DNA were analyzed by Illumina HiSeq 2500. Chromatin immunoprecipitation was performed for LDL1pro::LDL1:GFP-expressing Arabidopsis. ChIP DNA was analyzed by Illumina HiSeq 2500.