Comparison of Normalized Enrichment Scores (NES) from GSEA of human/zebrafish orthologous GSs (gene symbols described in GEO's GPL17670).

The list of unique genes with their corresponding fluorescence values from pooled lymphoid organs from 3 zebrafish per replica per phenotype (n = 4 replicas), was used for GSEA comparisons. The GSEA software was then applied to the Gene Sets (GSs) defined in the in-house microarray (gene symbols described in GEO's GPL17670, see S1 Table for a GS summary). GS Enrichment Scores (ES) were normalized for their number of genes (NES) and their significance was assessed by using 1000 gene permutations to estimate null distributions. The data were ordered from the highest to lowest NES of VHSVS. The differential expressions for the 3 phenotypes were calculated versus NI zebrafish. The remaining GSs did not show significant NES.
+, NES correlating with the first phenotype in the comparison.
-, NES correlating with NI in the comparison.
** (bold numbers), FDR q value < 0.05.
*, FDR q value <0.25.
bold, GSs containing multigene families.
Comparison of Normalized Enrichment Scores (NES) from GSEA of human/zebrafish orthologous GSs (gene symbols described in GEO's GPL17670).